Fig 1: TDP‐43 is elevated in ADEVs extracted from subjects with an autopsy‐confirmed diagnosis of LATE. Plasma concentrations of TDP‐43 were detected in NDEVs, ADEVs, and MDEVs (A‐F) and in EV depleted plasma (G,H) as measured by our in‐house ELISA and Cusabio kit. NDEV concentrations TDP‐43 was not significantly different between LATE‐NC (+) and LATE‐NC (–) subjects (A,D). ADE concentrations of TDP‐43 were significantly increased in LATE‐NC (+) as compared to LATE‐NC (–) subjects (B,E). MDEV concentrations of TDP‐43 were significantly increased in LATE ‐ NC (+), as measured by our in‐house ELISA (C), while MDE concentrations of TDP‐43 were significantly decreased in LATE‐NC (+), as measured by the Cusabio kit (F). EV depleted plasma concentrations of TDP‐43 were not significantly different between LATE‐NC (+) (n = 22) and LATE‐NC (–) subjects (n = 42).
Fig 2: Correlations between TDP‐43 accumulation in NDEVs, ADEVs, and MDEVs against cognition‐based variables including MMSE score and Braak NFT staging; sex; and APOEε4 carrier status as measured by our Cusabio kit. No correlation was observed with TDP‐43 accumulation in NDEVs, ADEs, and MDEs with a lower MMSE score (A–C) or Braak NFT stage (D–F). NDEV, ADE, and MDE concentrations of TDP‐43 were not significantly different in females versus males (G–I) or between APOEε4 carriers and non‐carriers (J–L). (n = 22, LATE‐NC (+); n = 42, LATE‐NC (–) subjects).
Fig 3: Correlations between TDP‐43 accumulation in NDEVs, ADVEs, and MDVEs against cognition‐based variables including MMSE score and Braak NFT staging; sex; and APOEε4 carrier status as measured by our in‐house ELISA. No correlation was observed with TDP‐43 accumulation in NDEVs, ADEVs, and MDEVs with a lower MMSE score (A–C) or Braak NFT stage (D–F). NDEV, ADEV, and MDEV concentrations of TDP‐43 were not significantly different between females versus males (G–I) or between APOEε4 carriers and non‐carriers (J–L). (n = 22, LATE‐NC (+); n = 42, LATE‐NC (–) subjects).
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